Lazio have Ciro Immobile and Pedro back, as they must beat FC Porto to reach the Europa League Round of 16, but Mehdi Taremi comes into the XI.
It kicks off at the Stadio Olimpico at 17.45 GMT.
Europa League Liveblog: Olympiacos-Atalanta, Lazio-Porto, Napoli-Barcelona
The Biancocelesti were beaten 2-1 in Portugal last week, so as the away goals rule has been abolished, that means they must win by two clear goals here to stay in the competition.
If the scores are level on aggregate, it goes to extra time and maybe even penalties.
Wilson Manafá and Fernando Andrade sit out bans.
There is a massive bonus for Lazio, as Immobile has recovered from flu symptoms after missing two games and is back in the starting XI.
Pedro has also surprisingly recovered from a knock to start, but Mattia Zaccagni suspended.
Francesco Acerbi and Manuel Lazzari are also on the treatment table.
Porto coach Sergio Conceicao brings in young talent Vitinha and hitman Mehdi Taremi after some surprising picks in the first leg, but sticks with Toni Martinez, who bagged a brace last time out.
Defender Wendell is suspended following an accumulation of yellow cards.
Lazio: Strakosha; Marusic, Luiz Felipe, Patric, Radu; Milinkovic-Savic, Leiva, Luis Alberto; Pedro, Immobile, Felipe Anderson
FC Porto: Diogo Costa; Bruno Costa, Pepe, Mbemba, Zaidu; Otavio, Uribe, Vitinha, Pepê; Taremi, Toni Martinez