This is a must-win match for Lazio after losing their Europa League opener, so Ciro Immobile, Felipe Anderson and Toma Basic start against Lokomotiv Moscow.
It kicks off at the Stadio Olimpico at 20.00 UK time (19.00 GMT).
The Biancocelesti boosted their confidence with a 3-2 Derby della Capitale win over Roma at the weekend, but the defeat to Galatasaray has made Europa League qualification an uphill struggle.
That loss in Turkey was decided by a Thomas Strakosha howler, but the goalkeeper retains the gloves in this competition.
Sarri rotates his squad only to a degree, keeping in big names like Pedro, Felipe Anderson and Immobile, giving Basic his first start.
Lokomotiv Moscow are not to be under-estimated, as they held Olympique Marseille to a 1-1 draw in their opening game.
Fedor Smolov has scored six goals in nine league games so far this season.
Lazio: Strakosha; Lazzari, Patric, Acerbi, Hysaj; Luis Alberto, Cataldi, Basic; Pedro, Immobile, Felipe Anderson
Lazio bench: Reina, Furlanetto, Luis Felipe, Escalante, Leiva, Akpa-Akpro, Romero, Milinkovic-Savic, Radu, Moro, Marusic, Muriqi
Lokomotiv Moscow: Guilherme; Zhivoglyadov, Barinov, Pablo, Rybchinskii; Zhemaletdinov, Maradishvili, Kulikov, Beka Beka; Anjorin, Smolov
Lokomotiv Moscow bench: Khudiakov, Savin, Kerk, Nenakhov, Kamano, Silyanov, Ivankov, Petrov, Babkin, Lisakovich
Ref: Pawson (ENG)