Federico Chiesa is confirmed in Italy’s attack and Emerson replaces injured Leonardo Spinazzola, while Spain look to Dani Olmo with Mikel Oyarzabal in the UEFA EURO 2020 semi-final, dropping Alvaro Morata.
It kicks off at Wembley Stadium at 20.00 UK time.
You can follow all the build-up and action as it happens and give your views on the LIVEBLOG.
The winners of this showdown go on to battle it out with England or Denmark for the trophy on Sunday.
Spinazzola is out for at least seven to eight months after surgery on his torn Achilles tendon, so Emerson Palmieri comes in at left-back.
Chiesa retains his place in the trident attack rather than Domenico Berardi, while Giorgio Chiellini again starts at centre-back in practically the same side that beat Belgium 2-1.
Lorenzo Insigne and Ciro Immobile complete the trio upfront, with Marco Verratti, Jorginho and Nicolò Barella in the middle.
Spain are without injured Pablo Sarabia, so Dani Olmo takes over in the front three with Mikel Oyarzabal and on-form Ferran Torres.
That means after defending him tooth and nail throughout the tournament, Luis Enrique drops Juventus striker Alvaro Morata to the bench.
Teenage midfielder Pedri has made quite an impact and partners Sergio Busquets and Koke.
Gerard Moreno is also dropped in favour of Eric Garcia.
Italy: Donnarumma; Di Lorenzo, Bonucci, Chiellini, Emerson Palmieri; Barella, Jorginho, Verratti; Chiesa, Immobile, Insigne
Italy bench: Sirigu, Meret, Locatelli, Belotti, Berardi, Pessina, Acerbi, Cristante, Bernardeschi, Bastoni, Florenzi, Toloi
Spain: Unai Simon; Azpilicueta, Eric Garcia, Laporte, Jordi Alba; Koke, Busquets, Pedri; Ferran Torres, Oyarzabal, Dani Olmo
Spain bench: De Gea, Sanchez, Diego Llorente, Pau Torres, Marcos Llorente, Morata, Gerard Moreno, Thiago Alcantara, Gaya, Rodri, Fabian Ruiz, Traore
Ref: Brych (GER)