Italy are expected to only make one forced change in the starting line-up against Spain, with Ciro Immobile starting up front at Wembley.
The match kicks off at 20.00 UK time.
The Azzurri and La Furia Roja are playing for a place in the Final of UEFA Euro 2020 and will continue what’s already a great rivalry of European football.
England or Denmark await in the Final, as the two clash tomorrow at the same stadium.
Roberto Mancini seems set to name the same line-up from the 2-1 win over Belgium in Munich, with only one forced change at left-back.
Leonardo Spinazzola underwent surgery in Finland yesterday and will be out for months, after suffering a ruptured Achilles at the Allianz Stadium last Friday.
Spain and Italy have a perfect balance in their head-to-head record, with 11 wins each and 15 draws in their 37 previous meetings.
The last match between the two sides came during the World Cup 2018 qualifiers, when La Furia Roja picked up a 3-0 win over Gian Piero Ventura’s men.
Alvaro Morata scored in that game, and the Juventus forward is expected to start in the middle of an attacking trident tonight as well.
Luis Enrique seems set to be without Pablo Sarabia, with Dani Olmo starting at left. Koke, Sergio Busquets and Pedri are set to start in the middle, for a battle with Marco Verratti, Jorginho and Nicolò Barella.
Italy (probable): Donnarumma; Di Lorenzo, Bonucci, Chiellini, Emerson; Verratti, Jorginho, Barella; Chiesa, Immobile, Insigne
Spain (probable): Unai Simon; Azpilicueta, Pau Torres, Laporte, Jordi Alba; Koke, Busquets, Pedri; Ferran Torres, Morata, Dani Olmo
Referee: Brych (GER)