Italy make one forced change, with Giovanni Di Lorenzo replacing Alessandro Florenzi, against a Switzerland side that includes Granit Xhaka and Xherdan Shaqiri.
It kicks off at the Stadio Olimpico in Rome at 20.00 UK time (19.00 GMT).
You can follow all the build-up and action as it happens and give your views on the LIVEBLOG.
The Azzurri got off to a strong Euro 2020 start by beating Turkey 3-0 with a Merih Demiral own goal, Ciro Immobile and Lorenzo Insigne on target.
That means a victory here would be enough to guarantee qualification into the Round of 16.
Roberto Mancini has to make one adjustment, as Alessandro Florenzi is injured, so Napoli full-back Di Lorenzo gets the nod rather than the more defensive Atalanta player Rafael Toloi.
The rest remains the same, with Manuel Locatelli filling in for injured Marco Verratti, then Domenico Berardi, Insigne and Immobile upfront.
Switzerland had a disappointing opener, drawing 1-1 with Wales, and were made to pay for not taking their chances.
There are several former and current Serie A players in the side, including Atalanta midfielder Remo Freuler, Torino’s Ricardo Rodriguez, Haris Seferovic (of Fiorentina, Lecce and Novara), ex-Inter midfielder Shaqiri and current Roma transfer target Granit Xhaka.
Even the coach, Vladimir Petkovic, is familiar from his years in charge of Lazio.
Italy: G Donnarumma; Di Lorenzo, Bonucci, Chiellini, Spinazzola; Barella, Jorginho, Locatelli; Berardi, Immobile, Insigne
Italy bench: Sirigu, Meret, Belotti, Pessina, Emerson, Chiesa, Acerbi, Cristante, Bernardeschi, Raspadori, Bastoni, Toloi
Switzerland: Sommer; Elvedi, Shär, Akanji; Mbabu, Freuler, Xhaka, Rodriguez; Shaqiri, Embolo; Seferovic
Switzerland bench: Mvogo, Kobel, Widmer, Zakaria, Vargas, Zuber, Sow, Fassnacht, Benito, Mehmedi, Gavranovic, Comert
Ref: Karasev (RUS)