Italy are transformed for their final Euro 2020 group game, including Marco Verratti, Andrea Belotti and Federico Chiesa, while Wales move to a False 9 approach.
It kicks off at the Stadio Olimpico in Rome at 17.00 UK time (16.00 GMT).
You can follow all the build-up and action as it happens and give your views on the LIVEBLOG.
The Azzurri have already qualified for the Round of 16 after beating Turkey and Switzerland 3-0, but need a point to guarantee top spot in Group A, as Wales have four points.
It would also allow them to match the all-time record of 30 games unbeaten, while they have not conceded a goal in 965 minutes of football.
Giorgio Chiellini is out injured and Alessandro Florenzi not fully fit, so Roberto Mancini makes radical changes to rotate the squad ahead of the knockout rounds.
That includes the introduction of Verratti, who has not even been on the bench for the first two games due to a knee injury he sustained with PSG last month.
Rafael Toloi, Emerson Palmieri, Matteo Pessina, Federico Bernardeschi and Andrea Belotti also step into the starting XI for the first time.
Federico Chiesa has a slight muscular issue, so is not risked from the start.
Wales have a full squad to choose from after their 1-1 draw with Switzerland and 2-0 victory against Turkey, achieved despite Bale missing a penalty.
Juventus midfielder Aaron Ramsey and Real Madrid’s Bale again provide the quality, with Cardiff City striker Kieffer Moore dropped in favour of a False 9 approach.
Italy: Donnarumma; Toloi, Bonucci, Bastoni, Emerson; Pessina, Jorginho, Verratti; Chiesa, Belotti, Bernardeschi
Italy bench: Sirigu, Meret, Di Lorenzo, Spinazzola, Locatelli, Castrovilli, Insigne, Acerbi, Cristante, Immobile, Barella, Raspadori
Wales: Ward; Ampadu, Rodon, Gunter; C Roberts, Morrell, Allen, N Williams; Bale, Ramsey, James
Wales bench: Hennessy, A Davies, B Davies, Lockyer, Wilson, T Roberts, Moore, Norrington-Davies, J Williams, Brooks, Mepham, Levitt
Ref: Hategan (ROM)