Napoli look to Dries Mertens, Arkadiusz Milik and Piotr Zielinski, while Stefan Lainer is ready to start for RB Salzburg in the Europa League.
The Round of 16 first leg tie kicks off at the Stadio San Paolo at 20.00 GMT.
You can follow all the build-up and action as it happens on the LIVEBLOG.
Napoli look to Dries Mertens, Arkadiusz Milik and Piotr Zielinski, while Stefan Lainer is ready to start for RB Salzburg in the Europa League.
The Round of 16 first leg tie kicks off at the Stadio San Paolo at 20.00 GMT.
You can follow all the build-up and action as it happens on the LIVEBLOG.
After losing to Juventus at the weekend and falling 16 points behind the Serie A leaders, this trophy remains the only realistic target for Carlo Ancelotti’s team this season.
This is why he uses a full-strength team, as Mario Rui returns from injury and Elseid Hysaj moves to his more habitual right-back role, leaving Kevin Malcuit on the bench.
The other change sees Lorenzo Insigne dropped in favour of Mertens and Milik, with support on the wings from Zielinski and Jose Callejon.
RB Salzburg have Lainer, Fredrik Gulbrandsen and Zlatko Junuzovic back in the squad after muscular problems, but Marin Pongracic is injured.
Lainer had been on the verge of a summer transfer to Napoli, but his club rejected all offers, so he now gets the chance to play at the Stadio San Paolo.
Munas Dabbur is partnered by Patson Daka rather than Takumi Minamino, while Dominik Szoboszlai has already attracted interest from Serie A for next season.
Both teams must be wary, because they have several players one yellow card away from suspension.
Napoli could lose Kalidou Koulibaly, Mario Rui and Nikola Maksimovic for the second leg, while RB Salzburg risk getting Lainer, Diadie Samassekou, Xaver Schlager and hitman Munas Dabbur banned.
Napoli: Meret; Hysaj, Maksimovic, Koulibaly, Mario Rui; Callejon, Allan, Fabian, Zielinski; Mertens, Milik
Napoli bench: Ospina, Chiriches, Malcuit, Ghoulam, Diawara, Ounas Insigne
RB Salzburg: Walke; Lainer, Ramalho, Onguene, Ulmer; Schlager, Wolf, Samassekou, Junuzovic; Daka, Dabbur
RB Salzburg bench: Stankovic, Vallci, Szoboszlai, Minamino, Gulbrandsen, Haland, Mwepu