Fiorentina leave out Sofyan Amrabat after a deadline day transfer row, but start Nico Gonzalez and Luka Jovic, while Torino have new signing Ivan Ilic on the bench in the Coppa Italia quarter-final.
It kicks off at the Stadio Artemio Franchi at 17.00 GMT.
You can follow all the build-up and action as it happens from this game and Roma-Cremonese on the LIVEBLOG.
A winner will be decided on the night, after 90 minutes, extra time or even penalties if necessary.
These teams met in Serie A on January 21, Toro winning 1-0 here with an Aleksey Miranchuk goal, their first victory in Florence since 1976.
Amrabat is left out by coach Vincenzo Italiano after he begged to join Barcelona on deadline day, seeing the loan with option to buy offer rejected.
Lucas Martinez Quarta, Gaetano Castrovilli and Riccardo Sottil are still on the treatment table.
Jovic is flanked by Nico Gonzalez and Christian Kouame, with Rolando Mandragora stepping into midfield.
Torino have new signing Ilic on the bench after his move from Hellas Verona, but the injury crisis drags on for David Zima, Ola Aina, Pietro Pellegri, Koffi Djidji and Valentin Lazaro.
The winners will face either Roma or Cremonese in the two-legged semi-final in April.
Fiorentina: Terracciano; Dodò, Milenkovic, Igor, Terzic; Bonaventura, Mandragora, Barak; Nico Gonzalez, Jovic, Kouame
Torino: Milinkovic-Savic; Schuurs, Buongiorno, Rodriguez; Singo, Ricci, Linetty, Vojvoda; Miranchuk, Vlasic; Sanabria