Lautaro Martinez and Edin Dzeko start in attack for Inter while Fiorentina boss Italiano picks Gaetano Castrovilli in central midfield for the Coppa Italia Final.
It kicks off at 20:00 GMT+1 at the Stadio Olimpico in Rome.
Salvatore Sirigu is the only player not available for the Tuscans, but he still made the trip to the Capital, while Inter are without the injured Henrikh Mkhitaryan and Milan Skriniar.
Gaetano Castrovilli starts in central midfield for La Viola alongside Sofyan Amrabat, so Rolando Mandragora initially goes on the bench. Arthur Cabral gets the nod up front, with Nico Gonzalez, Giacomo Bonaventura and Jonathan Ikoné behind the Brazilian.
Simone Inzaghi leaves Romelu Lukaku on the bench, opting for Lautaro Martinez and Dzeko up front. Marcelo Brozovic replaces Mkhitaryan as a deep-lying playmaker, with Nicolò Barella, Hakan Calhanoglu, Federico Dimarco and Denzel Dumfries completing a five-man midfield.
Inter are the defending Coppa champions, while the last time Fiorentina lifted the trophy was in 2000-01 under Roberto Mancini.
Fiorentina-Inter line-ups
Fiorentina (4-2-3-1): Terracciano; Dodò, Milenkovic, Martinez Quarta, Biraghi; Amrabat, Castrovilli; Ikoné, Bonaventura, Nico Gonzalez; Cabral.
Inter (3-5-2): Handanovic; Darmian, Acerbi, Bastoni; Dumfries, Barella, Brozovic, Calhanoglu, Dimarco; Lautaro Martinez, Dzeko.