Fiorentina start Arthur Cabral, Nico Gonzalez and Riccardo Sottil upfront, benching their new signings for the Conference League play-off against FC Twente.
It kicks off at the Stadio Artemio Franchi at 20.00 UK time (19.00 GMT).
You can follow all the build-up and action as it happens on the LIVEBLOG.
The second leg will be in the Netherlands next Thursday and the away goals rule does not apply, so if the teams end level on aggregate, they will go to extra time or even penalties.
This will see the Viola play in Europe for the first time since February 2017, earning this play-off after finishing seventh in Serie A last season.
Fiorentina are without injured Igor, Szymon Zurkowski and long-term absentee Gaetano Castrovilli, but compared to Sunday’s 3-2 Serie A win over Cremonese, there are a few changes.
Pietro Terracciano is chosen in goal, with Dodo and Rolando Mandragora again on the bench, joined by Luka Jovic, as Arthur Cabral gets the nod with Lorenzo Venuti, Alfred Duncan and Matija Nastasic.
FC Twente were fourth in the Eredivisie last term and have already started their season with four competitive games already, all of them victories, including the 7-2 aggregate win over Serbians Cukaricki in the last Conference League round.
Captain Wout Brama is out injured, picking up a muscular problem in mid-July, and it is not yet clear when he’ll be fit again.
Their star striker is Dutch veteran Ricky van Wolfswinkel.
Fiorentina: Terracciano; Venuti, Milenkovic, Nastasic, Biraghi; Duncan, Amrabat, Maleh; Nico Gonzales, Cabral, Sottil
FC Twente: Unnerstall; Brenet, Propper, Pleguezelo, Smal; Zerrouki, Vlap; Sadilek, Rots, Misidjan; Van Wolfswinkel
Ref: Stefanski (POL)