Luca Ranieri will make his Fiorentina debut this season against FK RFS, while Arthur Cabral is expected to start up front.
It kicks off at 17.45 UK time in Florence.
The Tuscans host the Latvians champion at the Franchi in the opening Conference League Group stage game tonight.
Gaetano Castrovilli, Nikola Milenkovic, Nico Gonzalez and Alfred Duncan are injured.
Italiano will rotate a bit, starting Jonathan Ikoné, Cabral and Riccardo Saponara up front. Ranieri, who is back from a successful loan spell at Salernitana last season, will start at the back and the same is true of Pierluigi Gollini in goal.
Italiano said yesterday that Fiorentina ‘won’t underestimate any opponent’ in the competition, although it’s still early to set a target for the Tuscans.
Fiorentina held Juventus to a 1-1 stalemate at the Stadio Franchi on Saturday finding the net for the first time in four games across all competitions.
They’ve scored only four goals in five Serie A games s far.
Conference League, Fiorentina-FK RFS: probable line-ups
Fiorentina (4-3-3): Gollini; Venuti, Quarta, Ranieri, Terzic; Bonaventura, Mandragora, Maleh; Ikone, Cabral, Saponara.
FK RFS: (4-2-3-1): Steinbors; Sorokins, Jagodinskis, Lipuscek, Mares; Jatta, Panic; Simkovic, Kouadio, Deocleciano; Ilic.