Juventus rely on Paulo Dybala, Cristiano Ronaldo and Mattia De Sciglio, while Ajax can start Frenkie de Jong in the Champions League quarter-final.
The first leg ended 1-1 and the decider kicks off at 20.00 UK time (19.00 GMT) in Turin.
You can follow the build-up and action as it happens and give your views on the LIVEBLOG.
Juventus rely on Paulo Dybala, Cristiano Ronaldo and Mattia De Sciglio, while Ajax can start Frenkie de Jong in the Champions League quarter-final.
The first leg ended 1-1 and the decider kicks off at 20.00 UK time (19.00 GMT) in Turin.
You can follow the build-up and action as it happens and give your views on the LIVEBLOG.
As the Bianconeri got an away goal in Amsterdam thanks to the diving Cristiano Ronaldo header, a goalless draw would be enough to send them through to face Tottenham or Manchester City in the semi-finals.
Douglas Costa, Mario Mandzukic, Giorgio Chiellini, Martin Caceres and Mattia Perin are out injured, so Daniele Rugani again steps in to defence with Alex Sandro rather than Leonardo Spinazzola.
The surprise twist is the presence of De Sciglio at right-back rather than Joao Cancelo, as although the Portugal international provided the assist for Ronaldo’s goal in the first leg, he was also at fault for the equaliser.
It’s reported the reason for that is Douglas Costa is not even fit enough for a spot on the bench, so Cancelo becomes the wild card option to be thrown on in the second half.
This does suggest a return to the system used against Atletico Madrid, with Emre Can dropping back to be an additional central defender when the full-backs push up.
Emre Can returns to the side after missing the first leg, with Dybala instead of Moise Kean leading the trident attack with Federico Bernardeschi and Ronaldo.
Dybala also becomes the captain in the absence of Chiellini and Mandzukic.
Ajax have left-back Nicolas Tagliafico suspended, but Noussair Mazraoui returns from his ban to take over in the back four.
Hassane Bande is still out of action, but Frenkie de Jonh is passed fit to play despite limping off 24 minutes into Saturday’s win over Excelsior with a hamstring problem.
Federico Bernardeschi and Blaise Matuidi are one yellow card away from suspension, with Donny van de Beek, Hakim Ziyech, Matthijs de Ligt and Daley Blind in the same position for Ajax.
Ajax have not beaten Juve since a 2-1 UEFA Cup Third Round result in December 1974, followed by three draws and eight wins, including two on penalty shoot-outs.
The most memorable was certainly the Champions League Final in Rome on May 22, 1996, when Juve last lifted this trophy.
The only two Ajax victories over Juventus were in 1973 (another Final for the Champions Cup) and 1974, held in Belgrade and Amsterdam.
Juventus: Szczesny; De Sciglio, Bonucci, Rugani, Alex Sandro; Can, Pjanic, Matuidi; Bernardeschi, Dybala, Ronaldo
Juventus bench: Pinsoglio, Barzagli, Cancelo, Khedira, Bentancur, Spinazzola, Kean
Ajax: Onana; Veltman, De Ligt, Blind, Mazraoui; Schöne, Van de Beek, De Jong; Ziyech, Tadic, Neres
Ajax bench: Varela, Sinkgraven, Huntelaar, Mahallan, Dolberg, De Wit, Ekkelenkamp
Ref: Turpin (FRA)