Italy defender Giorgio Chiellini suffered a problem in the flexor of his left thigh and will be examined in Florence tomorrow.
The Azzurri defender, who had a goal ruled out for handball in the 3-0 win over Switzerland on Wednesday, had to leave the pitch after 25 minutes with an injury.
The Juventus exited in agony and remained seated during the celebrations as Manuel Locatelli scored a brace and Ciro Immobile added a third to secure another three points at the Olimpico.
The Federcalcio has confirmed he suffered a muscular twinge in the left flexor, but the 36-year-old, who set a new outfield record with his 14th European Championship match, will undergo further examinations at Coverciano tomorrow.