Giorgio Chiellini makes his 14th European Championship appearance for Italy, a new record for outfield players.
The Azzurri take on Switzerland at the Stadio Olimpico in Rome, kicking off at 20.00 UK time (19.00 GMT).
You can follow all the build-up and action as it happens on the Liveblog.
Juventus defender Chiellini is in the starting XI, his 14th game in the European Championship finals.
That is more than any other Italy outfield player, surpassing Antonio Cassano, Alessandro Del Piero and Paolo Maldini on 13.
That trio are joined in second place by his Juventus teammate Leonardo Bonucci, who also starts this evening for his 13th appearance in the Euros.
Gianluigi Buffon remains out front with 17 matches in the European Championship finals for Italy.
The Nazionale won their Euro 2020 opener by beating Turkey 3-0.