With Berat Djimsiti and Rafael Toloi out of action, Merten De Roon could start in a three-man defence against Manchester United.
La Dea visit Old Trafford as the Group F leaders.
It kicks off at 20.00 UK time.
Youngsters Mattia Lovato and Giorgio Scalvini are available, but Gasperini could deploy the experienced Merten De Roon in a three-man defence or switch to a 4-3-1-2 system.
Ruslan Malinovskyi and Duvan Zapata rested against Empoli and entered the pitch in the second half, therefore are expected to start at Old Trafford tonight.
Former Serie A stars Diogo Dalot, Paul Pogba, Bruno Fernandes and Cristiano Ronaldo are all expected to start for Manchester United.
Manchester United vs. Atalanta: probable line-ups:
Manchester United (4-2-3-1): De Gea; Dalot, Lindelof, Maguire, Shaw; Pogba, McTominay; Greenwood, Bruno Fernandes, Cristiano Ronaldo; Rashford.
Atalanta (3-4-2-1): Musso; Palomino, Demiral, De Roon; Zappacosta, Koopmeiners, Freuler, Maehle; Pasalic, Malinovskyi; Zapata.
Man utd will take d 3 point to night utd_3 -1 Atalanta
Palomino is a weak link, and now they have a midfielder in the back 3. MU are a bit of a mess at the moment, so it’s a real shame Atalanta aren’t at full strength to take advantage. Given the group standings, a draw would be a good result.