Edin Dzeko and Lautaro Martinez lead Inter out at the Bernabeu with a reshuffled defence, while Real Madrid start Luka Jovic for injured Karim Benzema.
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Champions League Liveblog: Real Madrid-Inter and Milan-Liverpool
Both these sides have already qualified for the Round of 16, so it is a head-to-head for the top spot, which in theory ought to give them an easier ride in the draw for the knockout stages.
The only way Inter can win the group is with a victory at the Estadio Bernabeu.
Stefan de Vrij is available on the bench after his injury, but Mattia Darmian, Andrea Ranocchia and Joaquin Correa are out of action.
Danilo D’Ambrosio therefore completes the back three, with Denzel Dumfries and Ivan Perisic on the wings, Dzeko and Lautaro Martinez upfront.
Ancelotti’s Real Madrid are missing star striker Benzema, as he limped off with a muscular issue at the weekend, with Gareth Bale and Dani Ceballos already long-term absentees.
Jovic leads the attack with Rodrygo and Vinicius Jr.
When these sides met at San Siro, Inter had numerous chances cancelled out by Thibaut Courtois before losing to a late Madrid goal.
Real Madrid: Courtois; Carvajal, Militao, Alaba, F Mendy; Modric, Casemiro, Kroos; Rodrygo, Jovic, Vinicius
Real Madrid bench: Lunin, Fuidas, Vallejo, Nacho, Hazard, Asensio, Marcelo, Valverde, Lucas Vasquez, Isco, Mariano, Camavinga
Inter: Handanovic; D’Ambrosio, Skriniar, Bastoni; Dumfries, Barella, Brozovic, Calhanoglu, Perisic; Dzeko, Lautaro Martinez
Inter bench: Cordaz, Radu, Gagliardini, De Vrij, Alexis Sanchez, Vecino, Kolarov, Sensi, Vidal, Dimarco, Zanotti
Ref: Brych (GER)