Torino centre-back Alessandro Buongiorno is the surprise starter for Italy against Ukraine, receiving only the second cap of his career.
It kicks off on neutral turf at the BayArena in Leverkusen at 19.45 GMT.
The Azzurri can qualify for EURO 2024 with as little as a point, thanks to a superior head-to-head record after winning 2-1 when these sides met at San Siro in September.
Luciano Spalletti makes most of the changes in defence, where Giovanni Di Lorenzo returns after missing the 5-2 win over North Macedonia through suspension.
Federico Gatti played that game at centre-back, while Gianluca Mancini was mentioned as a possible option today.
The other was the Nations League Third Place Final on June 18, 2023, finishing in a 3-2 victory over the Netherlands.
His partner that day was again Francesco Acerbi, with Federico Dimarco at left-back.