Juventus are missing Giorgio Chiellini and Federico Bernardeschi, but are expected to start Paulo Dybala, Dusan Vlahovic and Alvaro Morata, while Atalanta are without several key players.
It kicks off on Sunday at 19.45 GMT at the Gewiss Stadium in Bergamo.
Max Allegri named his squad making the journey, which does not include injured Bernardeschi and Chiellini.
Moise Kean returns, as he was suspended for the midweek Coppa Italia 2-1 win over Sassuolo.
According to the latest reports, the coach does intend to repeat the all-attack formation that beat Verona last weekend, with Vlahovic flanked by Dybala and Morata.
Denis Zakaria should also get his third start in as many games since arriving from Borussia Monchengladbach.
As for Atalanta, they have goalkeeper Juan Musso suspended after a red card in the 2-1 home defeat to Cagliari.
Jose Luis Palomino, Duvan Zapata and Aleksei Miranchuk are injured.
Atalanta squad for Juventus: Dajcar, Rossi, Sportiello; Demiral, Djimsiti, Hateboer, Maehle, Pezzella, Scalvini, Toloi, Zappacosta; De Roon, Freuler, Koopmeiners, Malinovskyi, Pasalic, Pessina; Boga, Mihaila, Muriel
Juventus squad for Atalanta: Szczesny, Perin, Pinsoglio; Bonucci, Rugani, De Sciglio, De Ligt, Danilo, Cuadrado, Alex Sandro, Pellegrini; Arthur, Rabiot, McKennie, Locatelli, Zakaria; Vlahovic, Dybala, Morata, Kean, Kaio Jorge, Aké
Ecco i convocati per #AtalantaJuve!
⚫️⚪️ #FinoAllaFine#ForzaJuveBy @bitgetglobal pic.twitter.com/5K6uRDkPVy
— JuventusFC (@juventusfc) February 12, 2022