Francisco Conceicao, Kenan Yildiz and Douglas Luiz support Dusan Vlahovic as Juventus host VfB Stuttgart in the Champions League.
It kicks off at the Allianz Juventus Stadium in Turin at 20.00 UK time (19.00 GMT).
The Bianconeri won both opening European fixtures, beating PSV Eindhoven 3-1, then fighting back when down to 10 men for an incredible 3-2 victory away to RB Leipzig.
Michele Di Gregorio was sent off in that match and is therefore suspended, replaced by Mattia Perin in goal.
Nico Gonzalez, Teun Koopmeiners, Gleison Bremer and Arek Milik are injured, with Kenan Yildiz and Conceicao fanning out around Vlahovic.
Weston McKennie returns in midfield with Nicolò Fagioli and Danilo makes a rare start in defence.
Douglas Luiz was going to get his second consecutive start, but pulled out in the warm-up, so was replaced by Khephren Thuram.
VfB Stuttgart lost 3-1 to Real Madrid and drew 1-1 with Sparta Prague so far in the Champions League, but are coming off a 4-0 Bundesliga defeat to Bayern Munich at the weekend.
They have a very long injury list, including Chris Fuhrich, Dan-Axel Zagadou, Nikolas Nartey, Leonidas Stergiou and Luca Raimund.
On top of that, Wahid Faghir, Justin Diehl, Ramon Hendriks, Nick Woltemade and Stefan Drljaca are ineligible for this competition.
Coach Sebastian Hoeness makes some changes, bringing in Enzo Millot for Fabian Rieder and Max Mittelstadt in defence.
Juventus vs. VfB Stuttgart line-ups
Juventus: Perin; Savona, Kalulu, Danilo, Cabal; Fagioli, K Thuram, McKennie; Conceicao, Vlahovic, Yildiz
VfB Stuttgart: Nubel; Mittelstädt, Chabot, Rouault, Vagnoman; Stiller, Karazor; Leweling, Undav, Millot; Demirovic
Ref: Eskas (NOR)
