Luca Toni has a rare start alongside Alessandro Del Piero, while Cristiano Lucarelli and Giuseppe Mascara lead Napoli.
Tonight's 19.45 UK time kick-off sees the Bianconeri play their last chance of European football.
They must win this game and hope Roma lose at home to already-relegated Sampdoria.
It's a tough ask and protests are expected on Gigi Del Neri's final game on the Juve bench.
Toni is given a rare start with Del Piero, as Alessandro Matri is sent to the bench, while Marco Motta is suspended, so Giorgio Chiellini moves to left-back.
The injury list includes Fabio Quagliarella, Vincenzo Iaquinta, Felipe Melo, Momo Sissoko, Fabio Grosso and Zdenek Grygera.
Napoli still have Edinson Cavani on the naughty step, while Manuele Blasi is sidelined.
Ezequiel Lavezzi is also given a rest initially, allowing Mascara and veteran Lucarelli to lead the way.
There are also chances with Fabiano Santacroce and youngster Maiello given starts.
Juventus: Buffon; Salihamidzic, Barzagli, Bonucci, Chiellini; Krasic, Marchisio, Aquilani, Pepe; Del Piero, Toni
Napoli: De Sanctis; Santacroce, Cribari, Ruiz; Maggio, Gargano, Maiello, Zuniga; Sosa, Mascara; Lucarelli
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition - £5,000 monthly.