Lazio start Felipe Anderson, Miroslav Klose and Stefan de Vrij against Bayer Leverkusen in the Champions League play-off.
It kicks off in Rome at 19.45 UK time (18.45 GMT), follow all the action as it happens on our LIVEBLOG.
The second leg will be in Germany on August 26, the winners enter the Champions League group phase and losing side go into the Europa League instead.
Lazio start Felipe Anderson, Miroslav Klose and Stefan de Vrij against Bayer Leverkusen in the Champions League play-off.
It kicks off in Rome at 19.45 UK time (18.45 GMT), follow all the action as it happens on our LIVEBLOG.
The second leg will be in Germany on August 26, the winners enter the Champions League group phase and losing side go into the Europa League instead.
It has been a rough summer for the Aquile, who lost five consecutive pre-season matches, including the Italian Super Cup 2-0 to Juventus in Shanghai.
Coach Stefano Pioli performed a remarkable feat to snatch third place in Serie A last term and reached the Coppa Italia Final.
Tonight he is without Federico Marchetti, Filip Djordjevic, Edson Braafheid and suspended Stefan Radu.
Ogenyi ‘Eddy’ Onazi is picked ahead of Danilo Cataldi and Sergej Milinkovic-Savic in midfield, while Stefan de Vrij has recovered in time from a fever to lead the defence.
Felipe Anderson and Antonio Candreva complete the trident with veteran German Miroslav Klose.
Marco Parolo and Senad Lulic return, as they were banned for the Super Cup.
Bayer Leverkusen go into the Stadio Olimpico having already started the Bundesliga campaign, whereas Serie A doesn’t kick off until this weekend.
Omer Toprak and ex-Roma defender Tin Jedvaj are sidelined, but former Inter man Giulio Donati is on the bench for Coach Roger Schmidt.
The lead centre-forward is Stefan Kiessling, supported by Karim Bellarabi, Hakan Calhanoglu and Son Heung-Min.
The referee is Sweden’s Jonas Eriksson.
Lazio: Berisha; Basta, De Vrij, Mauricio, Lulic; Onazi, Biglia, Parolo; Candreva, Klose, Felipe Anderson
Lazio bench: Guerrieri, Konko, Gentiletti, Cataldi, Milinkovic-Savic, Keita, Kishna
Bayer Leverkusen: Leno; Hilbert, Papadopoulos, Tah, Wendell; Kramer, Bender; Bellarabi, Çalhanoğlu, Son; Kiessling
Bayer bench: Kresic, Donati, Ramalho, Kruse, Brandt, Aranguiz, Mehmedi