Fiorentina are without Juan Manuel Vargas, David Pizarro, Anderson, Mario Gomez and Marvin Compper against Milan.
It kicks off on Wednesday night at 19.45 GMT, click here for a match preview.
Vincenzo Montella has to deal with numerous absentees for the big game as he continues to target Napoli in third place.
Vargas and Compper both suffered muscular problems in training and have been ruled out of the squad.
They join Giuseppe Rossi, Gomez, Pizarro and ex-Manchester United midfielder Anderson on the treatment table.
Alessandro Matri and Massimo Ambrosini are available to face their former club.
Fiorentina squad for Milan: Ambrosini, Aquilani, Borja Valero, Bakic, Cuadrado, Diakite, Fazzi, Fernandez, Ilicic, Joaquin, Lupatelli, Matri, Neto, Pasqual, Rodriguez Gonzalo, Roncaglia, Rosati, Ryder, Savic, Tomovic, Venuti, Wolski
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition - £10,000 to be won!