Fiorentina and Napoli test their Scudetto credentials as Giuseppe Rossi goes head-to-head with Gonzalo Higuain.
It kicks off at 19.45 GMT, click here for a match preview.
The Viola are looking for a fourth straight win in all competition and again look to Rossi, Joaquin and Juan Guillermo Cuadrado.
Gonzalo Rodriguez is suspended, while Mario Gomez, Massimo Ambrosini, Josip Ilicic and Mati Fernandez miss out.
Napoli need a result to stay in second place and are still without the injured Juan Camilo Zuniga and Miguel Angel Britos.
Higuain leads the attack with support from Goran Pandev, Jose Maria Callejon and Dries Mertens.
Surprisingly, Marek Hamsik is rested as Rafa Benitez rotates his squad for this midweek round.
Lorenzo Insigne is again benched, while Federico Fernandez and Giandomenico Mesto are in defence.
Fiorentina: Neto; Roncaglia, Savic, Compper, Pasqual; Borja Valero, Pizarro, Aquilani; Cuadrado, Joaquin, Rossi
Napoli: Reina; Maggio, Albiol, Fernandez, Mesto; Inler, Behrami; Callejon, Pandev, Mertens; Higuain
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition - £11,000 to be won monthly!