The enticing grudge match Fiorentina-Juventus has Carlos Tevez and Fernando Llorente taking on Giuseppe Rossi and Juan Cuadrado.
It kicks off at the Stadio Artemio Franchi at 14.00 UK time (13.00 GMT), click here for a match preview.
The bitter rivalry between these clubs goes back a long way and has occasionally even spilled out on to the transfer market.
The Viola will be particularly fired up to get a victory, though injuries rule out Mario Gomez, Ante Rebic and Josip Ilicic.
Rossi leads the way with support from Cuadrado and Borja Valero, while David Pizarro has shaken off a knock to start with ex-Juventus midfielder Alberto Aquilani.
Juventus have different problems to contend with, as they are without Mirko Vucinic, Fabio Quagliarella, Stephan Lichtsteiner and Simone Pepe.
Arturo Vidal is left on the bench as punishment after he missed his flight back from Chile on international duty, returning to Turin only late Friday night.
Antonio Conte also has to consider the upcoming Champions League clash with Real Madrid on Wednesday evening.
Juve are unbeaten in Florence for the last 15 years, managing seven draws and four victories since Gabriel Batistuta sealed a 1-0 in December 1998.
Fiorentina: Neto; Roncaglia, Gonzalo, Savic; Pasqual, Ambrosini, Pizarro, Aquilani; Borja Valero, Cuadrado; Rossi
Fiorentina bench: Munua, Compper, Tomovic, Vecino, Bakic, Joaquin, Fernandez, Alonso, Wolski, Vargas, Matos, Iakovenko
Juventus: Buffon; Barzagli, Bonucci, Chiellini; Padoin, Pogba, Pirlo, Marchisio, Asamoah; Llorente, Tevez
Juventus bench: Storari, Rubinho, Motta, Caceres, Ogbonna, Peluso, Isla, De Ceglie, Bouy, Vidal, Giovinco
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition - £10,000 to be won!