Italy have called Manuel Pasqual and Lorenzo De Silvestri to replace the injured Ignazio Abate and Luca Antonelli.
Cesare Prandelli made the decision to bring in extra players after losing the two defenders during a 1-0 World Cup qualifying win over Bulgaria.
The Azzurri face the Czech Republic on Tuesday evening at the Juventus Stadium in Turin.
Fiorentina captain Pasqual and Sampdoria full-back De Silvestri have been called up and will join the squad on Saturday.
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