Lazio take on Maribor in the Europa League with Hernanes and Ederson starting together for the first time.
The Romans held Tottenham to a goalless draw at White Hart Lane in the first group game, so are looking to pick up their first win in the 18.00 UK time kick-off.
Hernanes and Ederson are paired together in midfield for the first time, while Sergio Floccari takes centre stage in attack.
They cannot count on Miroslav Klose, who is rested, but Mauro Zarate and Libor Kozak are ready to come off the bench.
Surprisingly, Albano Bizzarri is chosen in goal to rest Federico Marchetti.
Slovenians Maribor beat Panathinaikos in the first group game, so are not to be underestimated.
The only changes from that game see Nejc Potokar ruled out by injury and Zeljko Filipovic preferred to Dejan Mezga.
Brazilian Arghus is suspended for this fixture.
Lazio: Bizzarri; Konko, Ciani, Dias, Cavanda; Cana; Candreva, Gonzalez, Hernanes, Ederson; Floccari
Lazio bench: Marchetti, Biava, Scaloni, Onazi, Mauri, Zarate, Kozak
Maribor: Handanovic; Milec, Rajcevic, Vidovic, Mejac; Cvijanovic, Filipovic, Mertelj, Ibraimi; Tavares, Beric
Maribor bench: Pridigar, Lesjak, Mezga, Dodlek, Trajkovski, Komazec, Ploj
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition - £10,000 to be won!